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Fig. 1 | Diabetology & Metabolic Syndrome

Fig. 1

From: Multiply restimulated human cord blood-derived Tregs maintain stabilized phenotype and suppressive function and predict their therapeutic effects on autoimmune diabetes

Fig. 1

Fold expansion and purity of UCB-Tregs expanded for 18 and 42 days in vitro. (A) Fold expansion; n = 18 (d18), n = 13 (d42). (B) Representative images showing gating strategy for the immunophenotyping of Tregs stained with CD3, CD4, CD25, CD127, and CD8. (C) Percentage of Tregs (CD3+CD4+CD25+CD127− cells); n = 3 (d0), n = 15 (d18), and n = 13 (d42) and CD8+ T cells (CD3+CD8+ cells); n = 3 (d0), n = 15 (d18), and n = 13 (d42). Percentage of CD25+CD127− T cells in CD8+ T cells; n = 15 (d18), n = 13 (d42). Purity among d0, d18, and d42 Tregs was compared using the Kruskal–Wallis test, followed by Dunn’s multiple comparison test. Comparison of percentage of CD25+CD127− T cells in CD8+ T cells was analyzed using Mann-Whitney test. **p < 0.01, ns indicates no significant difference

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Diabetology & Metabolic Syndrome

ISSN: 1758-5996

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